Journal: Experimental Neurobiology
Article Title: The Effects of Acute Stress on Evoked-cortical Connectivity through Wide-field Optical Mapping of Neural and Hemodynamic Signals
doi: 10.5607/en23009
Figure Lengend Snippet: In vivo wide-field optical mapping (WFOM) of the excitatory neural calcium transient signal and hemodynamic response evoked by a whisker stimulation. (A) Experimental scheme for in vivo wide-field optical mapping and blood sampling for ELISA with acute stress protocol. (B) Plasma corticosterone level in control and stressed mice (Mann-Whitney U test, control: n=6; stress: n=6). (C) Seeds from the cortical regions based on the anatomical structure: #1 & #8: Primary motor (M1), #2 & #9: Secondary motor cortex (M2), #3 & #10: Primary somatosensory Forelimb (S1FL), #4 & #11: secondary somatosensory (S2), #5 & #12: Retrosplenial cortex (Ret), #6 & #13: Primary visual cortex (V1), #7 & #14: Primary somatosensory barrel field (S1BF). (D, E) Excitatory neural calcium fluorescence signal and (F, G) hemodynamic reflectance response during sensory stimulation in the left primary and secondary somatosensory cortex from each seed ROIs. Orange color shaded area refers the stimulation period (10~14s). (H) Quantified calcium fluorescence measurements, including onset, peak-value, time-to-peak, area under curve (AUC), in control and stressed mice for seed #11 and #14. (I) Quantified hemodynamics in control and stressed mice for seed #11 and #14. (J) (Left) The images of calcium fluorescence at 14s, and (Right) the fluorescence alteration of cortical projection by acute stress at 12, 14, and 16s (Image acquired by subtraction, stress-control). (K) (Left) The images of hemodynamics responses at 14s, and (Right) the hemodynamic alteration of cortical projection by acute stress at 12, 14, and 16s (Image acquired by subtraction, stress-control).
Article Snippet: An ELISA kit (Assaypro) was used to measure the corticosterone concentration in the plasma.
Techniques: In Vivo, Whisker Assay, Sampling, Enzyme-linked Immunosorbent Assay, Control, MANN-WHITNEY, Fluorescence